Western blot analysis was performed on whole cell extracts (30 µg lysate) of COS-7 (Lane 1), MDCK (Lane 2), C2C12 (Lane 3), MDA-MB-231 (Lane 4), PC-12 (Lane 5), RSC96 (Lane 6), tissue extracts of Mouse Lung (Lane 7) and Rat Stomach (Lane 8). The blot was probed with beta Tubulin Loading Control Monoclonal Antibody (BT7R), DyLight 680 (Product # MA5-16308-D680, 1 µg/mL). A 50 kDa band corresponding to beta Tubulin was observed across the cell lines and tissues tested. Known quantity of protein samples were electrophoresed using Novex® NuPAGE® 10% Bis-Tris gel (Product # NP0322BOX), XCell SureLock™ Electrophoresis System (Product # EI0002) and Novex® Sharp Pre-Stained Protein Standard (Product # LC5800). Resolved proteins were then transferred onto a nitrocellulose membrane with iBlot® 2 Dry Blotting System (Product # IB21001). The membrane was probed with the relevant primary antibody following blocking with 5 % skimmed milk. Fluorescent detection was performed using the Odyssey® Fc imaging system (Li-cor Biosciences).